Journal: Cancer immunology research

Article Title: The conventional dendritic cell 1 subset primes CD8 + T cells and traffics tumor antigen to drive anti-tumor immunity in the brain

doi: 10.1158/2326-6066.CIR-22-0098

Figure Lengend Snippet: (A) GBM patient with pre-administered 5-ALA: tumor visualized under white (top) or blue (bottom) light. (B) PPIX as tumor antigen surrogate, traceable in tumor-infiltrating APCs. (C) PPIX expression in a bulk unstained tumor from a 5-ALA-resected GBM (left) or in U343 cells treated with 5-ALA (right) (representative of independent experiments). (D-E) CD3ε vs. PPIX expression of live/CD45+ cells from patient-derived PBMCs or resected GBM tumors. (F-G) PPIX+ APC subsets across 3 GBM tumors (two primary, one recurrent) compared to patient PBMCs. (H) Tumor-infiltrating PPIX+ APCs vs. T-cells. Data representative of eight patients (six primary, two recurrent) in which GBM and matched intraoperative PBMCs were taken. Plotted are mean +/- SEM. **p<0.01, ***p<0.001, ****p<0.0001, ns: not significant. Unpaired two-tailed T tests for (E) and (H). Paired two-tailed T tests for (G).

Article Snippet: U343 cells were obtained from The Cancer Cell Line Encyclopedia at the Broad Institute in 2014 [ 50 ].

Techniques: Expressing, Derivative Assay, Two Tailed Test

Journal: Cytotechnology

Article Title: Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

doi: 10.1007/s10616-014-9839-4

Figure Lengend Snippet: IC 50 (µg/ml) of different extract/fraction in HeLa and U343 cells by SRB assay after 48 h of exposure

Article Snippet: Human epithelial cervical carcinoma cell line (HeLa), human lung adenocarcinoma (A549), human breast adenocarcinoma (MCF7), human normal breast epithelium cells (HBL-100), human neuronal glioblastoma (U343) were procured from National Centre for Cell Sciences (Pune, Maharashtra, India) and cultured with Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma-Aldrich Co. LLC, St. Louis, MO, USA) supplemented with 10 % Fetal Bovine Serum (FBS) (HiMedia Laboratories, Mumbai, India) and 1 × Penicillin/Streptomycin at 37 °C in CO 2 incubator (NU-5501E/G, NuAire Inc., Plymouth, MN, USA) in humidified atmosphere of 5 % CO 2 and 95 % air.

Techniques: Sulforhodamine B Assay

Journal: Cytotechnology

Article Title: Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

doi: 10.1007/s10616-014-9839-4

Figure Lengend Snippet: Percentage apoptotic cells in three different cancer cell lines. All values are mean ± SEM of 100 cells. a p < 0.05 versus HeLa control, b p < 0.05 versus U343 control, c p < 0.05 versus A549 control

Article Snippet: Human epithelial cervical carcinoma cell line (HeLa), human lung adenocarcinoma (A549), human breast adenocarcinoma (MCF7), human normal breast epithelium cells (HBL-100), human neuronal glioblastoma (U343) were procured from National Centre for Cell Sciences (Pune, Maharashtra, India) and cultured with Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma-Aldrich Co. LLC, St. Louis, MO, USA) supplemented with 10 % Fetal Bovine Serum (FBS) (HiMedia Laboratories, Mumbai, India) and 1 × Penicillin/Streptomycin at 37 °C in CO 2 incubator (NU-5501E/G, NuAire Inc., Plymouth, MN, USA) in humidified atmosphere of 5 % CO 2 and 95 % air.

Techniques: Control

Journal: Cytotechnology

Article Title: Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

doi: 10.1007/s10616-014-9839-4

Figure Lengend Snippet: Comet parameter in three different cell lines

Article Snippet: Human epithelial cervical carcinoma cell line (HeLa), human lung adenocarcinoma (A549), human breast adenocarcinoma (MCF7), human normal breast epithelium cells (HBL-100), human neuronal glioblastoma (U343) were procured from National Centre for Cell Sciences (Pune, Maharashtra, India) and cultured with Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma-Aldrich Co. LLC, St. Louis, MO, USA) supplemented with 10 % Fetal Bovine Serum (FBS) (HiMedia Laboratories, Mumbai, India) and 1 × Penicillin/Streptomycin at 37 °C in CO 2 incubator (NU-5501E/G, NuAire Inc., Plymouth, MN, USA) in humidified atmosphere of 5 % CO 2 and 95 % air.

Techniques: Control

Journal: Oncotarget

Article Title: Inhibition of 13-cis retinoic acid-induced gene expression of reactive-resistance genes by thalidomide in glioblastoma tumours in vivo

doi:

Figure Lengend Snippet: A. Volume growth as function of time during treatment. The treatment of animals with RA or THAL as a sole compound did not affect xenograft growth in comparison to untreated controls. Treatment with RA in combination with THAL caused significant growth delay of tumours in comparison to control or animals which were treated with RA or THAL as sole compounds. Mean values and standard errors are shown. The effect of RA and THAL on cell proliferation in vitro B, C. The treatment of U251 (B) or U343 (C) with RA inhibited cell proliferation in comparison to untreated cells. The treatment with THAL as a sole compound did not affect cell proliferation in comparison to untreated controls but a significant sensitizing effect of THAL in combination with RA ( P = 0.0007 and P = 0.015 for U251 and U343, respectively for days 4–10) was observed in comparison to cells treated with RA only.

Article Snippet: The U251 and U343 cell lines (obtained from ATCC, LGC Promochem, Wesel, Germany, and the tumour bank of the German Cancer Research Centre, Heidelberg, Germany, respectively) were seeded in 12-well plates (Falcon, Corning, Wiesbaden, Germany) at 1 × 10 4 cells per well and incubated in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS; both from Biochrom, Berlin, Germany) under 5% CO 2 at 37°C.

Techniques: Comparison, Control, In Vitro